Hoechst stained hela cells
Nettet14. apr. 2024 · a HeLa cells treated with 0.5 mM arsenite (AS) for 30 min or 0.3 M sorbitol for 30 min were immunostained for UBAP2L together with G3BP1, TIAR, eIF4E, or PABPC1 (scale bar = 10 μm).b HeLa cells ... NettetA flow cytometric live-dead cell assay that uses the dual staining of Hoechst 33342 and propidium iodide (HO-PI) was evaluated for its ability to determine the clonogenicity of treated HeLa cells. The colony-forming assay was used as the reference to determine the capability of the HO-PI assay to me …
Hoechst stained hela cells
Did you know?
NettetBy staining intact cells with the DNA binding, vital, fluorescent dye, Hoechst 33342 (H0342), it is possible to sort viable (clonogenic) cells according to DNA content and thereby obtain cells from the G 1 S, and G 2 phases of the cell cycle. Sorted HeLa cells were used to study cell cycle-dependent DNA supercoiling ability using the halo assay ... Nettet9. nov. 2024 · Image 1 (above): Actin filament staining in HeLa cells. Actin filaments (green) were stained with Phalloidin-iFluor 488 reagent (ab176753); tubulin filaments were stained with a mouse anti-tubulin antibody/goat anti-mouse IgG (red). Nuclei were stained with Hoechst 33342. Image 2 (above): Actin filament staining in HeLa cells.
NettetCCDC7 Activates Interleukin-6 and Vascular Endothelial Growth Factor to Promote Proliferation via the JAK-STAT3 Pathway in Cervical Cancer Cells Javascript is currently disabled in your browser. Several features of this site will not function whilst javascript is … NettetHeLa (/ ˈ h iː l ɑː /; also Hela or hela) is an immortalized cell line used in scientific research. It is the oldest and most commonly used human cell line . [1] The line is derived from cervical cancer cells taken on February 8, 1951, [2] from Henrietta Lacks , a 31-year-old African-American mother of five, who died of cancer on October 4, 1951, and after …
NettetImages and charts from Hoechst-stained HeLa cells after 20 hours of Camptothecin treatment. (A) Image analysis of Camptothecin-induced cytotoxicity with green borders outlining Hoechst-stained nuclei. Dose-response curves show the cytotoxic effects of Camptothecin on Hoechst-determined (B) cell count and (C) occupied surface area. Nettet20. jan. 2007 · Human cancer cells, specifically HeLa cells, with DNA stained blue. The central and rightmost cell are in interphase. The cell on the left is going through mitosis and its chromosomes have condensed.
NettetHoechst Dye Cell Culture Adipocytes Cell Biology Molecular Cell Biology 28th Mar, 2024 Adam M. Passman Barts Cancer Institute Looks like mycoplasma to me. In my experience it has to be...
Nettet1. Culture cells in an appropriate medium and vessel for fluorescence microscopy. 3. Remove the medium. 4. Add sufficient staining solution to cover the cells. 5. Incubate for 5–10 minutes, protected from light. 6. Optional: You may image directly in the staining solution, if you wish. 7. Remove the staining solution. 8. Wash the cells 3 ... cpot validationNettet31. jul. 2024 · Moreover, endogenous cleavage was also detected in a panel of tumor cell lines. Results Compared with the control, both the LDH release and PI/Hoechst double-staining experiments suggested that SKN induced perforation and enhancement of the permeability of the cell membranes that resulted in pyroptosis in … magnetic specsNettetDisplaying low-cytotoxic effects and increased cell permeability, Hoechst stains are ideal for live- and fixed-cell fluorescent DNA staining and nuclei imaging techniques, such as cell counting, automated DNA determination, cell cycle analysis, chromosome sorting, and nuclear counterstaining. cpot total scoreNettetFeatures of Hoechst 33342 Fluorescent Stain: • Hoechst dye —blue fluorescent stain specific for DNA (i.e., nuclei of eukaryotic cells) • Convenient —provided as an easy-to-use Hoechst dye solution (20 mM) • Cellular imaging —cell-permeable dye is effective for fixed-cell and live-cell staining. • Counterstain —ideal for use ... cpot ventilatedNettetUsually mycoplasma is quite well visible with a normal exposure, with nucleus well visible with clear borders and the additional staining from mycoplasma more concentrated at the cell borders,... cpo tundraNettetI am trying to stain nuclei of Hela cells using Hoechst 33342. I have tried several working concentrations ranging from 0.2 ug/mL to 1 ug/mL for minimum 2 mins up to 10 mins. I obtain a... c potter\u0027sNettetFrom left to right: (A) HeLa cells—nuclei are stained with Hoechst 33258 (colored blue) and cell membranes were stained with CellMask Green (colored green). ( B ) Fluorescently labeled polymer–peptide conjugate at 50 μM concentration at 37 °C (specifically TAT12 ) after 2 h incubation. magnetic speed controller