WebThe method used will depend on the experiment and the information required. For easy setup, with PI staining of DNA content for flow cytometry we recommend our Propidium … WebPropidium iodide (PI, Sigma) BMG LABTECH microplate reader. Cerebellar granule cells were obtained from post-natal Wistar rats (P5-8) and cultured directly onto 24-well plates at a plating density of 280-300,000 cells per well. PI was diluted with dH 2 O to a concentration of 5 mg/mL and used at a final concentration of 50 μg/mL (diluted in ...

Protocol - Propidium Iodide Cell Cycle Staining Protocol

WebPropidium iodide (PI) is a popular red-fluorescent nuclear and chromosome counterstain. Since propidium iodide is not permeant to live cells, it is also commonly used to detect dead cells in a population. PI … WebPI is a well-accepted marker for degenerating cells when applied prior to fixation (pre-fixation PI staining). Here, PI was added to the sections after the fixation (post-fixation PI … smoked cheeseburger fatty

FITC Annexin V Apoptosis Detection Kit I - BD Biosciences

Webstaining to remove residual buffer salts from the slide. Air dry. before counter-This final rinse will help reduce nonspecific background staining on the glass. Counterstaining Protocol 5.1 Make a 1.5 µM PI staining solution by diluting the 1 mg/mL (1.5 mM) stock solution 1:1000 in PBS. Pipet 300 µL of this staining solution directly onto the ... WebApr 24, 2011 · The ability of PI to enter a cell is dependent upon the permeability of the membrane; PI does not stain live or early apoptotic cells due to the presence of an intact … rivers hunt